2. Cell Cycle/DNA Damage Autophagy
  3. DNA Alkylator/Crosslinker Autophagy DNA/RNA Synthesis
  4. Carboplatin

Carboplatin  (Synonyms: 卡铂; NSC 241240)

目录号: HY-17393 纯度: 99.96%
COA 产品使用指南

摩尔计算器

Carboplatin (NSC 241240) 是一种 DNA 合成 抑制剂，其与 DNA 结合，抑制复制和转录并诱导细胞死亡。Carboplatin (NSC 241240) 是顺铂的衍生物，是一种有效的抗癌剂。

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Carboplatin Chemical Structure

Carboplatin Chemical Structure

CAS No. : 41575-94-4

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规格 价格 是否有货 数量
50 mg ￥450
In-stock
100 mg ￥640
In-stock
200 mg ￥1150
In-stock
500 mg ￥2070
In-stock
1 g   询价  
5 g   询价  

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Customer Review

Other Forms of Carboplatin:

Carboplatin 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 48 篇科研文献

IHC
WB

    Carboplatin purchased from MCE. Usage Cited in: Cancer Lett. 2018 Aug 16;436:75-86.  [Abstract]

    FTY720+Tamoxifen and FTY720+Carboplatin increase apoptosis marker, cleaved caspase 3 in a PDX model of EOC. IHC of tumor sections is used to detect the apoptosis marker cleaved caspase 3 and the cell cycle inhibitor p21.

    Carboplatin purchased from MCE. Usage Cited in: Genome Med. 2016 Oct 31;8(1):116.  [Abstract]

    Phosphorylation level of RPS6 upon Temsirolimus treatment. The effective target engagement is confirmed in Temsirolimus-treated tumors by showing reduced phosphorylation of downstream mTOR targets, RPS6 and 4EBP1, and an associated increase in autophagy (LC3A/B).

    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Carboplatin (NSC 241240) is a DNA synthesis inhibitor which binds to DNA, inhibits replication and transcription and induces cell death. Carboplatin (NSC 241240) is a derivative of CDDP and a potent anti-cancer agent.

    IC50 & Target

    DNA Alkylator[1]
    体外研究
    (In Vitro)

    Carboplatin 是一种抗肿瘤剂，在亲核试剂和人乳腺癌 MCF-7 细胞胞质提取物存在的情况下具有增强的 DNA 结合活性[1]。
    Carboplatin 对人卵巢细胞如 A2780、SKOV3、IGROV1 和 HX62 的细胞毒性低于 17-AAG，IC50 分别为 6.177、12.442、2.233 和 116.068 μM。此外，Carboplatin 不影响 HSP90 或改变 17-AAG 的活性以抑制 HSP90[2]。
    Carboplatin 降低 Brca1 (IC50，3.4 μM) 和 Brca2 细胞 (IC50，1.9 μM) 的活力。Carboplatin (25 μM) 与 ABT-888 结合也显示出对 BRCA1 细胞的凋亡作用[3]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Carboplatin 相关抗体:
    体内研究
    (In Vivo)

    Carboplatin (60 mg/kg，腹腔注射) 对肿瘤有一定影响，但与 17-AAG 联合使用可显著抑制携带 A2780 人卵巢癌异种移植物的小鼠的肿瘤生长[2]。
    Carboplatin (25 mg/kg，口服) 与 ABT-888 联合使用可延缓 Brca2 异种移植物中的肿瘤生长[3]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    分子量

    371.25

    Formula

    C6H12N2O4Pt
    CAS 号
    性状

    固体

    颜色

    White to off-white

    中文名称

    卡铂；碳铂
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    Please store the product under the recommended conditions in the Certificate of Analysis.
    溶解性数据
    细胞实验： 

    H2O 中的溶解度 : 8.33 mg/mL (22.44 mM; 超声助溶; DMSO can inactivate Carboplatin's activity)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 2.6936 mL 13.4680 mL 26.9360 mL
    5 mM 0.5387 mL 2.6936 mL 5.3872 mL
    查看完整储备液配制表

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂： PBS

      Solubility: 10 mg/mL (26.94 mM); 澄清溶液; 超声助溶 (<60°C)

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
    纯度 & 产品资料

    纯度: 99.96%

    COA (293 KB) HNMR (237 KB) RP-HPLC (250 KB) 元素分析报告 (216 KB)

    产品使用指南 (1538 KB)
    参考文献
    Cell Assay
    [2]

    Exponentially growing A2780 cells are plated in 96 well microtitre plates. For experiments studying the effect of sequence of exposure to 17-AAG or Carboplatin, cells are exposed to increasing concentrations of 17-AAG or Carboplatin for 24 h. A period of 24-h exposure to the first agent is chosen so that the A2780 cells will be exposed to the first drug for at least one doubling time (18-24 h). The cells are then washed with sterile phosphate buffered saline and the medium is replenished. Following this, the second drug (to which the cells are not exposed to in the first 24 h) or medium is added for 96 h. SRB assays are carried out. All experiments are carried out in triplicate[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [2]

    Mice[2]
    The A2780 human ovarian cancer cell line is grown as a subcutaneous xenograft in female athymic NCr nude mice (nu/nu) by injecting 4 × 106 cells in each flank. Mice with established tumors corresponding to a mean volume of 0.69 mm3 are randomized into groups (six animals each) for treatment with either control vehicle (43% ethanol, 33% polypropylene glycol and 24% cremaphor diluted 1:7 with sterile water) days 1-4, 17-AAG (80 mg/kg intraperitonially, days 1-4), Carboplatin (60 mg/kg IP day 0) or a combination of 17-AAG (80 mg/kg IP days 1-4) and Carboplatin (60 mg/kg IP day 0). Tumor growth is assessed three times weekly and tumor volumes are calculated according to a validated formula: volume = 4.19 × (a/4 + b/4)3, where a is the longer and b the shorter diameter. Tumor volumes are then expressed as a proportion of the volume at the start of treatment (relative tumor volume)[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    参考文献

    Carboplatin 相关分类

    完整储备液配制表

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 2.6936 mL 13.4680 mL 26.9360 mL 67.3401 mL
    5 mM 0.5387 mL 2.6936 mL 5.3872 mL 13.4680 mL
    10 mM 0.2694 mL 1.3468 mL 2.6936 mL 6.7340 mL
    15 mM 0.1796 mL 0.8979 mL 1.7957 mL 4.4893 mL
    20 mM 0.1347 mL 0.6734 mL 1.3468 mL 3.3670 mL

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Carboplatin41575-94-4NSC 241240卡铂碳铂NSC241240NSC-241240DNA Alkylator/CrosslinkerAutophagyDNA/RNA SynthesisInhibitorinhibitorinhibit

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    目录号：
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